Molecular Identification of Entamoeba histolytica from Diarrhetic Patients in Baghdad Province, Iraq

Abstract

This research used molecular detection tools to identify the amoebiasis-causing Entamoeba histolytica in individuals with diarrhea. We collected stool samples from one hundred individuals exhibiting diarrheal symptoms and categorized them based on their gender and age. Ninety percent of the samples contained entamoeba germs, according to the results of a direct microscope examination. Microscopic examination revealed that females had a much higher rate of Entamoeba infection (86.73%) than males (70.8%), and this difference was significant at the P value threshold of 0.05. Significant infection rates were also found in the age groups of 5–11 years and over 50 years (85.23 and 78.81%), respectively, whereas the age group of more than 2 years had a lower infection rate (43.45%). Using the small subunit gene, the nested multiplex polymerase chain reaction demonstrated that the infection rate for E. histolytica 91 was higher (91 percent). Compared to men (82.35%), females had a much greater prevalence of Entamoeba histolytica infection (89.23%). The infection rate was 100% attributed to E. histolytica in both the younger age group (28–48 years) and the elderly age group (>2). The percentage of individuals with Entamoeba histolytica infection was determined by nested multiplex PCR findings, gender, and age.